Changes in the Isoelectric Focusing Profile of Pituitary Follicle-stimulating Hormone in the Developing Male Rat 1

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Abstract
Pituitary glands, hypothalami and trunk blood were obtained from male rats at 5, 15, 18, 21 and 29 days of age, on the day of balnopreputial separation (days 42-45), and during aduthood. The forms of FSH present with each pituitary were separted by polyacrylamide gel isoelectric focusing. Serum and pituitary gonadotropins, hypothalamic LHRH and the profile of FSH forms across the isoelectric focusing gel were determined by radioimmunoassay. No change in the relative proportions of FSH forms were observed between 5 and 21 days of age. Likewise, only slight changes in serum and pituitary gonadotropin levels and hypothalamic LHRH content were observed at these times. After 21 days of age, dramatic increases in serum and pituitary gonadotropon levels were observed. Similarly, a shift in FSH forms within the pituitary to more basic and bioactive forms as observed at this time. Apparently, during the transition through puberty in the male rat, not only the absolute amount, but also the isoelectric focusing profile, of FSH change.

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