ENHANCEMENT OF MURINE ERYTHROID COLONY FORMATION IN THE PRESENCE OF ACTIVATED LYMPHOCYTES-T
- 1 January 1980
- journal article
- research article
- Vol. 95 (6) , 934-942
Abstract
Erythroid colony formation was enhanced by treatment of mouse bone marrow cells with PHA [phytohemagglutinin] prior to culture in diffusion chambers implanted into intact mice. Addition of PHA-pretreated thymocytes, lymph node lymphocytes and spleen cells to untreated bone marrow cells also resulted in increased numbers of erythroid colonies in diffusion chambers but had no effect on myeloid colonies. PHA did not augment erythroid colony formation when bone marrow was depleted of Thy 1-bearing cells, although cells capable of erythroid differentiation were still present as judged by the response to erythropoietin. Mixing of bone marrow depleted of Thy 1 + cells with PHA-treated thymocytes restored augmented erythroid colony formation. Lack of responsiveness of bone marrow cells to concanavalin A and pokeweed mitogen may indicate that a specific T [thymus-derived] cell subpopulation reacting with PHA is able to stimulate erythropoiesis. Addition of untreated thymocytes, lymph node lymphocytes and spleen cells into cultures had no effect.This publication has 10 references indexed in Scilit:
- GROWTH OF MOUSE AND HUMAN BONE MARROW IN DIFFUSION CHAMBERS IN MICE.Cell Proliferation, 1979
- Human erythroid burst-forming unit: T-cell requirement for proliferation in vitro.The Journal of Experimental Medicine, 1978
- EFFECT OF GLUCAN, A MACROPHAGE ACTIVATOR, ON MURINE HEMATOPOIETIC CELL-PROLIFERATION IN DIFFUSION-CHAMBERS IN MICE1978
- Pure and mixed erythroid colony formation in vitro stimulated by spleen conditioned medium with no detectable erythropoietin.Proceedings of the National Academy of Sciences, 1977
- THE ROLE OF ERYTHROPOIETIN IN REGULATION OF POPULATION SIZE AND CELL CYCLING OF EARLY AND LATE ERYTHROID PRECURSORS IN MOUSE BONE MARROWCell Proliferation, 1977
- FACTORS CONTROLLING STEM-CELL RECIRCULATION .3. EFFECT OF THYMUS ON MIGRATION AND DIFFERENTIATION OF HEMATOPOIETIC STEM-CELLS1977
- THYMUS DEPENDENCY OF BONE-MARROW STEM-CELL PROLIFERATION IN RESPONSE TO CERTAIN ANTIGENS1976
- ASSESSMENT OF ERYTHROCYTIC AND GRANULOCYTIC COLONY FORMATION IN AN INVIVO PLASMA CLOT DIFFUSION CHAMBER CULTURE SYSTEM1976
- Effect of Thymectomy on Hematopoietic Organs of the Opossum "Embryo."Experimental Biology and Medicine, 1965
- A Direct Measurement of the Radiation Sensitivity of Normal Mouse Bone Marrow CellsRadiation Research, 1961