Purification and Characterization of Lecithin:Cholesterol Acyltransferase
- 1 January 1978
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 38 (sup150) , 1-5
- https://doi.org/10.3109/00365517809104891
Abstract
The purification of lecithin:cholesterol acyltransferase (LCAT) from human plasma is reported. Hydroxylapatite fractions were approximately 16,000 fold purified over the starting plasma and were free of high-density lipoprotein (HDL) and albumin. The enzyme showed one band on polyacrylamide gel electrophoresis, SDS-urea polyacrylamide gel electrophoresis, isoelectric focusing, and one arc in immunodiffusion against a goat antiserum preparation. It was determined to be a glycoprotein with a molecular weight of approximately 70,000 and a pi of 3.7-4.0.Keywords
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