Baculovirus-infected cells do not produce the amyloid peptide of Alzheimer's disease from its precursor
- 1 July 1996
- journal article
- Published by Wiley in FEBS Letters
- Vol. 389 (2) , 157-161
- https://doi.org/10.1016/0014-5793(96)00561-3
Abstract
The amyloid peptide (Aβ) of Alzheimer's disease (AD) is produced by proteolytic cleavage of a larger precursor, the amyloid peptide precursor or APP. The discovery of pathogenic mutations in the APP gene provides strong evidence for the hypothesis that APP metabolism is involved in the etiology of AD. To study the metabolism of the protein, human APP has been expressed in several mammalian cell types. Insect cells, infected by a recombinant baculovirus carrying the human APP sequence, also provide an interesting expression system because these cells do not produce endogenous APP. Baculovirus-infected cells synthesize very high amounts of extracellular soluble APP, after cleavage of the transmembrane protein, as described for mammalian cells. However, we demonstrate here that insect cells do not produce Aβ from APP. These results suggest that while the enzymatic activity needed for the production of soluble APP is conserved between insect and mammalian cells, the enzymes required for the production of Aβ from APP are only expressed in mammalian cellsKeywords
This publication has 28 references indexed in Scilit:
- Candidate Gene for the Chromosome 1 Familial Alzheimer's Disease LocusScience, 1995
- Cloning of a gene bearing missense mutations in early-onset familial Alzheimer's diseaseNature, 1995
- Release of Excess Amyloid β Protein from a Mutant Amyloid β Protein PrecursorScience, 1993
- Mutation of the β-amyloid precursor protein in familial Alzheimer's disease increases β-protein productionNature, 1992
- A pathogenic mutation for probable Alzheimer's disease in the APP gene at the N–terminus of β–amyloidNature Genetics, 1992
- Presenile dementia and cerebral haemorrhage linked to a mutation at codon 692 of the β–amyloid precursor protein geneNature Genetics, 1992
- A mutation in the Amyloid Precursor Protein Associated with Hereditary Alzheimer's DiseaseScience, 1991
- Early-onset Alzheimer's disease caused by mutations at codon 717 of the β-amyloid precursor protein geneNature, 1991
- Segregation of a missense mutation in the amyloid precursor protein gene with familial Alzheimer's diseaseNature, 1991
- The precursor of Alzheimer's disease amyloid A4 protein resembles a cell-surface receptorNature, 1987