Translation of chloroplast-encoded mRNA: potential initiation and termination signals
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (5) , 2057-2080
- https://doi.org/10.1093/nar/17.5.2057
Abstract
A survey of 196 protein-coding chloroplast DNA sequences demonstrated the preference for AUG and UAA codons for initiation and termination of translation, respectively. As in prokaryotes at every nucleotide position from -25 to +25 (AUG is +1 to +3) and for 25 nucleotides 5'' and 3'' to the termination codon an A or U is predominant, except for C at +5 and G at +22. A Shine-Dalgarno (SD) sequence (GGAGG or tri- or tetranucleotide variant) was found within 100 bp 5'' to the AUG codon in 92% of the genes. In 40% of these cases, the location of the SD sequence was similar to that of the consensus for prokaryotes (-12 to -7 5'' to AUG), presumed to be optimal for translation initiation. A SD sequence could not be located in 6% of the chloroplast sequences. We propose that mRNA secondary structures may be required for the relocation of a distal SD sequences to within the optimal region (-12 to -7) for initiation of translation. We further suggest that termination at UGA codons in chloroplast genes may occur by a mechanism, involving 16S rRNA secondary structure, which has been proposed for UGA termination in Escherichia coli.This publication has 110 references indexed in Scilit:
- Mutant 16S ribosomal RNA: a codon-specific translational suppressor.Proceedings of the National Academy of Sciences, 1988
- The soybean chloroplast genome: complete sequence of therps19 gene, including flanking parts containing exon 2 ofrpl2 (upstream), but lackingrpl22 (downstream)Nucleic Acids Research, 1988
- Evidence for in vivo trans splicing of pre-mRNAs in tobacco chloroplastsCell, 1987
- Nucleotide sequence of the gene for ribosomal protein S11 in pea chloroplast DNANucleic Acids Research, 1987
- Spinach plastid genes coding for initiation factor IF-1, ribosomal protein S11 and RNA polymerase -subunitNucleic Acids Research, 1986
- Two partially homologous adjacent light-inducible maize chloroplast genes encoding polypeptides of the P700 chlorophyll a-protein complex of photosystem I.Journal of Biological Chemistry, 1985
- Nucleotide sequence and evolution of the 18S ribosomal RNA gene in maize mitochondriaNucleic Acids Research, 1984
- Translational control of IS10 transpositionCell, 1983
- Location and nucleotide sequence of the gene for the proton-translocating subunit of wheat chloroplast ATP synthaseProceedings of the National Academy of Sciences, 1982
- Structures of the genes for the β and ε subunits of spinach chloroplast ATPase indicate a dicistronic mRNA and an overlapping translation stop/start signalProceedings of the National Academy of Sciences, 1982