Effect of paradichlorobenzene on the somatic chromosomes and mitosis of Lens esculenta (L.) Moench.

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Abstract
Effect of paradichlorobenzene PDB on the somatic chromosomes and mitosis of L. esculenta (L.) Moench. var. microsperma was studied after giving prolonged treatment of 4-48 h under controlled conditions. The germiability and growth was inversely proportional to amount and duration of PDB treatment. Various types of mitotic and chromosomal anomalies were observed. These included contraction and condensation of chromosomes, precocious separation of chromatids, chromasomal fragmentations, chromosome bridges, tetraploid cells, binucleate cells, etc. Chromosome breaks were generally restricted to secondary constrictions, satellites, heterochromatic regions and rarely to centromere regions. The potentialities of PDB as a mutagenic chemical were emphasized.