Polymorphism of human pituitary lutropin (LH)

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Abstract
The complete microheterogeneous system of human pituitary lutropin was demonstrated by gel isoelectric focusing. In addition 7 LH isohormones could be isolated by preparative column focusing and characterized with respect to physicochemical, biological and immunological properties. The in vivo biopotencies ranged from 4.50–11.50 IU/mg, the in vitro bioactivity was from 1.20–10.10 IU/mg, and the immunological activity was from 3.10–7.55 IU/mg. The sialic acid content was found to be 1.8–3.2%. Treatment with neuraminidase resulted in a shift of all bands to the alkaline region, however the 7 LH forms were still present.