NUTRITIONAL REQUIREMENTS OF FRAXINUS CALLUS CULTURES
- 1 March 1966
- journal article
- research article
- Published by Wiley in American Journal of Botany
- Vol. 53 (3) , 263-269
- https://doi.org/10.1002/j.1537-2197.1966.tb07333.x
Abstract
A satisfactory synthetic medium has been developed for continuous growth of Fraxinus pennsylvanica Marsh. callus cultures. The medium contains modified Reinert and White (1956) inorganic nutrient solution with 5 mg/liter Fe as NaFe‐EDTA and supplemented with myoinositol 10 mg/liter, pyridoxine HCl 0.1 mg/liter, 2,4‐dichlorophenoxyacetic acid 0.04 mg/liter, kinetin 1 mg/liter, sucrose 20 g/liter, and agar 10 g/liter. myo‐Inositol, pyridoxine and an auxin are essential. α‐Naphthaleneacetic acid is an effective alternate auxin. Kinetin and to some extent gibberellic acid improve the yields. Thiamine has no effect.Funding Information
- Wisconsin Alumni Research Foundation
- National Science Foundation (G‐5890, G‐24038)
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