Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris
Open Access
- 14 February 2008
- journal article
- research article
- Published by Springer Nature in BMC Biotechnology
- Vol. 8 (1) , 1-12
- https://doi.org/10.1186/1472-6750-8-14
Abstract
Background: Rhipicephalus (Boophilus) spp. ticks economically impact on cattle production in Africa and other tropical and subtropical regions of the world. Tick vaccines constitute a cost-effective and environmentally friendly alternative to tick control. The R. microplus Bm86 protective antigen has been produced by recombinant DNA technology and shown to protect cattle against tick infestations.Results: In this study, the genes for Bm86 (R. microplus), Ba86 (R. annulatus) and Bd86 (R. decoloratus) were cloned and characterized from African or Asian tick strains and the recombinant proteins were secreted and purified fromP. pastoris. The secretion of recombinant Bm86 ortholog proteins inP. pastorisallowed for a simple purification process rendering a final product with high recovery (35–42%) and purity (80–85%) and likely to result in a more reproducible conformation closely resembling the native protein. Rabbit immunization experiments with recombinant proteins showed immune cross-reactivity between Bm86 ortholog proteins.Conclusion: These experiments support the development and testing of vaccines containing recombinant Bm86, Ba86 and Bd86 secreted inP. pastorisfor the control of tick infestations in Africa.Keywords
This publication has 50 references indexed in Scilit:
- Vaccination of cattle with TickGARD induces cross-reactive antibodies binding to conserved linear peptides of Bm86 homologues in Boophilus decoloratusVaccine, 2007
- The Known Distribution and Ecological Preferences of the Tick Subgenus Boophilus (Acari: Ixodidae) in Africa and Latin AmericaExperimental and Applied Acarology, 2006
- Polymorphism of the bm86 Gene in South American Strains of the Cattle Tick Boophilus microplusExperimental and Applied Acarology, 2005
- Integrated control of Boophilus microplus ticks in Cuba based on vaccination with the anti-tick vaccine Gavac TMExperimental and Applied Acarology, 2004
- Mapping of B-cell epitopes in the N-terminal repeated peptides of Anaplasma marginale major surface protein 1a and characterization of the humoral immune response of cattle immunized with recombinant and whole organism antigensVeterinary Immunology and Immunopathology, 2004
- Immunization of cattle with synthetic peptides derived from the Boophilus microplus gut protein (Bm86)Veterinary Immunology and Immunopathology, 2002
- Immunological Control of Ticks through Vaccination with Boophilus microplus Gut AntigensAnnals of the New York Academy of Sciences, 2000
- Adjuvant and immunostimulating properties of the recombinant Bm86 protein expressed in Pichia pastorisVaccine, 1998
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976