Interaction of site specific hirudin variants with α‐thrombin
- 29 February 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 229 (1) , 87-90
- https://doi.org/10.1016/0014-5793(88)80803-2
Abstract
The kinetics of complex formation between recombinant hirudin or recombinant hirudin mutants with thrombin were analyzed. In order to elucidate the inhibitor's reactive site peptide bond predetermined amino acid substitutions were introduced at positions of basic amino acid residues by means of site-directed mutagenesis of a hirudin gene. In comparison to recombinant hirudin (K i = 19 pM) only those mutant inhibitors which were modified at amino acid position Lys47 showed a higher K i value for their complexes with thrombin. The observed effects are mainly due to increased k off rate constants.Keywords
This publication has 17 references indexed in Scilit:
- [57] Sequencing end-labeled DNA with base-specific chemical cleavagesPublished by Elsevier ,2004
- Antithrombin properties of C‐terminus of hirudin using synthetic unsulfated Nα‐acetyl‐hirudin45–65FEBS Letters, 1987
- Kinetics of the inhibition of thrombin by hirudinBiochemistry, 1986
- Expression, secretion and processing of hirudin in E. coli using the alkaline phosphatase signal sequenceFEBS Letters, 1986
- Chemical Synthesis and Expression of a Gene Coding for Hirudin, the Thrombin-Specific Inhibitor from the LeechHirudo medicinalisBiological Chemistry Hoppe-Seyler, 1986
- Kinetic Studies on the Interaction of α1-Proteinase Inhibitor (Pittsburgh) with Trypsin-Like Serine ProteinasesBiological Chemistry Hoppe-Seyler, 1986
- [32] Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectorsPublished by Elsevier ,1983
- The slow-binding and slow, tight-binding inhibition of enzyme-catalysed reactionsTrends in Biochemical Sciences, 1982
- [13] ProthrombinPublished by Elsevier ,1976
- Activation of prothrombin with (taipan snake) venomThrombosis Research, 1973