The mechanism of the alkaline phosphatase reaction: insights from NMR, crystallography and site‐specific mutagenesis
Open Access
- 22 November 1999
- journal article
- review article
- Published by Wiley in FEBS Letters
- Vol. 462 (1-2) , 7-11
- https://doi.org/10.1016/s0014-5793(99)01448-9
Abstract
The proposed double in‐line displacement mechanism of Escherichia coli alkaline phosphatase (AP) involving two‐metal ion catalysis is based on NMR spectroscopic and X‐ray crystallographic studies. Th...Keywords
This publication has 43 references indexed in Scilit:
- Kinetic and X-ray structural studies of three mutant E. coli alkaline phosphatases: insights into the catalytic mechanism without the nucleophile ser102 1 1Edited by D. C. ReesJournal of Molecular Biology, 1998
- Kinetic and Structural Consequences of Replacing the Aspartate Bridge by Asparagine in the Catalytic Metal Triad ofEscherichia coliAlkaline PhosphataseJournal of Molecular Biology, 1996
- Mutations at Positions 153 and 328 inEscherichia coliAlkaline Phosphatase Provide Insight Towards the Structure and Function of Mammalian and Yeast Alkaline PhosphatasesJournal of Molecular Biology, 1995
- Alkaline Phosphatase Is an Almost Perfect EnzymeBiochemistry, 1994
- SETOR: Hardware-lighted three-dimensional solid model representations of macromoleculesJournal of Molecular Graphics, 1993
- Reaction mechanism of alkaline phosphatase based on crystal structuresJournal of Molecular Biology, 1991
- Alteration of aspartate 101 in the active site of Escherichia coli alkaline phosphatase enhances the catalytic activityProtein Engineering, Design and Selection, 1989
- Function of arginine-166 in the active site of Escherichia coli alkaline phosphataseBiochemistry, 1988
- Stereochemistry of phosphoryl group transfer using a chiral [16O, 17O, 18O] stereochemical course of alkaline phosphataseNature, 1978
- Effect of magnesium on the properties of zinc alkaline phosphataseBiochemistry, 1977