Chronological Changes in the Accumulation of Poly(A)+ RNA in Developing Cells of Xenopus laevis, with Special Reference to Primordial Germ Cells. (in situ hybridization poly (A)+ RNA PGCs Xenopus)

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Abstract
Chronological changes in the accumulation of poly(A)+RNA in developing cells of X. laevis were traced by in situ hybridization with 3H-poly(U) as a probe. Almost all the somatic cells acquired 3H-poly(U) binding sites by 3.5 days after fertilization (stage 44). At this stage, the primordial germ cells (PGC), which had just moved to the genital ridges from the deep endodermal cell mass showed moderate 3H-poly(U) binding activity. The Ag grains disappeared from the PGC after stage 47 (6 days after fertilization), while the somatic cells, which constituted the neural tube, genital ridges, kidney and intestine exhibited strong 3H-poly(U) binding activity. Activity for 3H-poly(U) binding reappeared in the PGC after stage 52 (21 days after fertilization) when sexual differentiation of gonads became detectable morphologically. The chronological accumulation and disappearance of 3H-poly(U) binding activity in the PGC in contrast to the constant activity in somatic cells is discussed.