Adjuvant Action of Poly A:U on T and B Rosette-Forming Cells in SRBC-Immunized Mice

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Abstract
The adjuvant action of complexes of polyadenylic and polyuridylic acids (poly A:U) on the primary immune response to SRBC was studied with respect to its effect on the production of thymus-derived (T) and bone marrow-derived (B) rosette-forming cells (RFC). RFC were assayed by two separate methods; one in which rosettes were prepared by incubation at 37 °C and then at 4 °C, the other by centrifugation at 4 °C. Two to three times more RFC were detected in mice receiving SRBC only when RFC were assayed by the incubation method. Most RFC detected by the incubation method were Θ-negative (BRFC) whereas 15–32% of the RFC detected by the centrifugation method were Θ -positive (TRFC). Both methods detected a 2- to 3-fold amplification of BRFC in mice receiving SRBC plus poly A:U. However, while the centrifugation method detected a 2- to 3-fold increase in T RFC, the incubation method detected a greater than 10-fold amplification of T RFC.

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