DEMONSTRATION OF INTRATUMORAL INFILTRATION OF TUMOR-SPECIFIC LYT-1+2- T-CELLS MEDIATING DELAYED-TYPE HYPERSENSITIVITY RESPONSE AND INVIVO PROTECTIVE IMMUNITY

  • 1 February 1986
    • journal article
    • research article
    • Vol. 77  (2) , 182-189
Abstract
The present study demonstrates the intratumoral infiltration of lymphocytes mediating anti-tumor delayed-type-hypersensitivity (DTH) responses as well as in vivo protective immunity. The surface phenotype and tumor specificity of these effector lymphocytes were determined. X5563 tumor-infiltrating lymphoid cells were obtained from the tumor mass of syngeneic C3H/HeN mice 2 weeks after the intradermal inoculation of 106 viable X5563 tumor cells. These lymphoid cells consisted of Thy-1-positive (29-35%), surface immunoglobulin-positive (16-29%), large granule-positive (15-25%) and esterase-staining-positive (10-20%) cells. They were tested for anti-X5563 DTH responses by utilizing a local adoptive transfer system and for tumor-neutralizing activity in a Winn assay. The results indicate that X5563 DTH responses and conveyed complete protection against the tumor. Treatment of these lymphoid cells with anti-Thy-1.2 or anti-Lyt antibodies plus complement revealed tha the in vivo anti-tumor immune responses were mediated predominantly by a Lyt-1+2-T cell subset. Such Lyt1+2-T cell-mediated immunity was tumor-specific, since X5563-infiltrating and syngeneic MH134 hepatoma-infiltrating cells exhibited DTH response and tumor-neutralizing activity selectively against the respective tumor cell types. Thus, these results indicate that tumor-specific in vivo-protective Lyt-1+2-T cells infiltrate into the tumor mass. The results are discussed in the context of 1) the interrelation of DTH responses and the tumor protection mediated by the Lyt-1+2-T cell subset and 2) possible cellular interactions between Lyt-1+2-T cells and co-existing nonspecific tumoricidal effector cells such as macrophages.