MONOAMINE-CONTAINING CELLS IN THE GASTRIC MUCOSA OF THE KITTEN: A NEW METHOD EMPLOYING COMPARATIVE FLUORESCENCE AND ELECTRON MICROSCOPY ON IDENTICAL CELLS

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Abstract
Monoamine-containing cells and adrenergic nerve fibers are clearly demonstrated by fluorescence microscopy on semi-thin sections of Araldite-embedded materials previously fixed with a mixture of formaldehyde and glutaraldehyde. Identical monoamine-containing structures were examined by both fluorescence microscopy and EM. Fluorescence microscopy reveals 2 kinds of monoamine-containing cells in the fundic mucosa of the kitten: one with yellow fluorescence and the other with blue-green fluorescence. By EM, yellow fluorescent cells are EC [enterochromaffin] cells containing characteristic polymorphous secretory granules, while blue-green fluorescent cells have round secretory granules and are probably A, X and 3rd type cells.