Alpha and beta spore occurrence in Phomopsis juniperovora pycnidia on Juniperus virginiana and in culture

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Abstract
Pycnidia of Phomopsis juniperovora on Juniperus virginiana were examined for occurrence of beta spores. Beta spores were observed in only 3% of the pycnidia on diseased material collected in the field. The most beta spores observed in one pycnidium was four. Thirty-two percent of the pycnidia produced after diseased material was collected contained beta spores. The beta spore content in induced pycnidia ranged from 0 to 85% of the spores observed. The pycnidia observed from cultures on potato dextrose agar contained between 15 and 98% beta spores. The absence of beta spores in pycnidia on field-collected material hinders proper diagnosis of the causal organism.

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