EFFECTS OF RECOMBINANT HUMAN-TUMOR NECROSIS FACTOR-ALPHA, RECOMBINANT HUMAN GAMMA-INTERFERON, AND PROSTAGLANDIN-E ON COLONY FORMATION OF HUMAN HEMATOPOIETIC PROGENITOR CELLS STIMULATED BY NATURAL HUMAN PLURIPOTENT COLONY-STIMULATING FACTOR, PLURIPOIETIN-ALPHA, AND RECOMBINANT ERYTHROPOIETIN IN SERUM-FREE CULTURESH

  • 1 September 1986
    • journal article
    • research article
    • Vol. 46  (9) , 4357-4361
Abstract
The influences of pure human pluripotent colony-stimulating factor, highly purified pluripoietin .alpha., pure recombinant human tumor necrosis factor .alpha., pure recombinant human .gamma.-interferon, and natural prostaglandin E1 (PGE1) were evaluated on colony formation of multipotential and erythroid progenitor cells in the presence of recombinant erythropoietin and hemin and on colony formation of granulocyte-macrophage progenitors in normal human marrow cultured in the presence or absence of serum. Serum was replaced by bovine serum albumin, iron-saturated transferrin, cholesterol, and calcium chloride. Increasing concentrations of pluripotent colony-stimulating factor and pluripoietin .alpha. stimulated increasing numbers of colonies from nonadherent low-density T-lymphocyte-depleted cells in the absence and presence of serum. Growth was usually greater in the presence of serum and on a unit basis pluriopoietin .alpha. was more active than pluripotent colony-stimulating factor. Recombinant human tumor necrosis factor .alpha. and recombinant human .gamma.-interferon suppressed colony formation colony forming unit-granulocyte-macrophage, burst forming unit-erythroid, and colony forming unit-granulocyte-erythroid-macrophage-megakaryocyte; PGE1 suppressed colony formation by colony-forming unit-granulocyte-macrophage, stimulated colony formation by burst forming unit-granulocyte-macrophage, stimulated colony formation by burst forming unit-erythroid, and had no effects on colony formation by colony forming unit-granulocyte-erythroid-macrophage-megakaryocyte in both serum-contianing and serum-free medium. The PGE1 enhancing effects on erythroid colony formation required T-lymphocytes. Thus, results are similar using serum-containing and serum-free cultures of human bone marrow cells and serum-free defined culture medium can be used to study the mechanisms of action of purified natural and recombinant growth and suppressor molecules in vitro.