Crystallization and Preliminary X-Ray Crystallographic Study of Streptomyces olivaceoviridis E-86 -Xylanase
- 1 May 1997
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 121 (5) , 826-828
- https://doi.org/10.1093/oxfordjournals.jbchem.a021659
Abstract
β-Xylanase from Streptomyces olivaceoviridis E-86 has been crystallized by the hanging drop vapor diffusion method from 25% saturated ammonium sulfate and 2% McIlvaine buffer, pH 5.7. The crystals diffract to at least 1.9 Å resolution, and belong to space group P212121, with unit-cell dimensions of a=79.6 Å, b=95.2 Å, and c=140.3 Å. There are probably two xylanase molecules (MW=45 K) per asymmetric unit.Keywords
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