IN VITRO REGENERATION OF SHOOTS ON STEM EXPLANTS OF HAPLOID AND DIPLOID FLAX (LINUM USITATISSIMUM)

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Abstract
Shoots were regenerated from stem explants of haploid (2n = x = 15) and diploid (2n = 2x = 30) flax (L. usitatissimum L. cv. ''Rocket'' 4) on a modified Murashige and Skoog medium supplemented with benzyladenine and napthaleneacetic acid. Segments of 15 mm cultured from donor plants at early flowering, regenerated 6.1 (mean) shoots per mm which were epidermal in origin and retained the chromosome number of the donor. The rate and frequency of shoot regeneration was greater at 30.degree. C than 25.degree. C. Callus originated from the cut ends of explants and was more vigorous at the lower temperature. Shoots of callus origin were infrequent and had the doubled chromosome complement (2n = 2x = 30 and 2n = 4x = 60).