Comparison of micropuncture and microcatheterization in papillary collecting duct

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Abstract
Collecting duct transport of fluid, Na and K was studied in rats infused with Ringer solution (5 ml .cntdot. 100 g body wt-1). A terminal segment of surface collecting duct in the exposed papilla was catheterized as far upstream as possible under visual observation. After fluid sampling the same duct was punctured at the same site with a glass micropipette and a 2nd sample was taken. Samples were obtained from the opening of the duct at the papilla tip by catheter and micropipette. No significant difference between the 2 collection sites was found in the fraction of filtered Na, K or fluid remaining in the tubule, independent of the sampling technique used, indicating that volume expansion inhibited salt and water reabsorption. Although fractional fluid and Na remainders were slightly higher and K remainder lower in upstream micropuncture samples compared to catheterization samples, the good correlation between collections obtained with both techniques suggests that both are equally valid as indicators of transport in terminal collecting ducts.

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