Sequence differences between mouse embryonic y- and adult .beta.-globin messenger ribonucleic acids
- 19 August 1980
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 19 (17) , 3967-3973
- https://doi.org/10.1021/bi00558a012
Abstract
Poly(A)-containing RNA was isolated from yolk sac cells of BDF1 hybrid mice on the 12th day of gestation. In vitro translation of the poly(A)-containing RNA by a mRNA dependent reticulocyte lysate system revealed that this RNA contained a high concentration of mRNAs from 2 embryonic globin chain subunits, .gamma.-1 and .gamma.-2. Cross hybridization of complementary DNA (cDNA) to the embryonic globin mRNAs with adult reticulocyte globin mRNAs and with cloned cDNAs for adult .alpha.- and .beta.-globin chain mRNAs showed that the sequence of embryonic globin .gamma.-chain mRNA differed from that of adult .beta.-chain mRNA.This publication has 7 references indexed in Scilit:
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