Isolation of a heparan sulfate-containing proteoglycan from basement membrane.

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Abstract
A unique, basement membrane proteoglycan was isolated from the [mouse] Engelbreth-Holm-Swarm (EHS) sarcoma. This proteoglycan, estimated to be 0.75 .times. 106 daltons, contained about equal amounts of protein and covalently linked heparan sulfate. Antibody prepared against this proteoglycan reacts with the basement membrane matrix in the tumor and with the basement membranes in skin, kidney and cornea. The heparan sulfate proteoglycan is apparently a normal constituent of basement membranes that presumably plays an important role in the organization of basement membrane components and that also may determine the permeability of basement membranes to acidic molecules.