Jaagsiekte Sheep Retrovirus Envelope Efficiently Pseudotypes Human Immunodeficiency Virus Type 1-Based Lentiviral Vectors
Open Access
- 1 March 2004
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 78 (5) , 2642-2647
- https://doi.org/10.1128/jvi.78.5.2642-2647.2003
Abstract
Jaagsiekte sheep retrovirus (JSRV) infects lung epithelial cells in sheep, and oncoretroviral vectors bearing JSRV Env can mediate transduction of human cells, suggesting that such vectors might be useful for lung-directed gene therapy. Here we show that JSRV Env can also efficiently pseudotype a human immunodeficiency virus type 1-based lentiviral vector, a more suitable vector for transduction of slowly dividing lung epithelial cells. We created several chimeric Env proteins that, unlike the parental Env, do not transform rodent fibroblasts but are still capable of pseudotyping lentiviral and oncoretroviral vectors.Keywords
This publication has 80 references indexed in Scilit:
- Putative Phosphatidylinositol 3-Kinase (PI3K) Binding Motifs in Ovine Betaretrovirus Env Proteins Are Not Essential for Rodent Fibroblast Transformation and PI3K/Akt ActivationJournal of Virology, 2003
- Role of Virus Receptor Hyal2 in Oncogenic Transformation of Rodent Fibroblasts by Sheep Betaretrovirus Env ProteinsJournal of Virology, 2003
- Mechanism of Cell Entry and Transformation by Enzootic Nasal Tumor VirusJournal of Virology, 2002
- A Phosphatidylinositol 3-Kinase Docking Site in the Cytoplasmic Tail of the Jaagsiekte Sheep Retrovirus Transmembrane Protein Is Essential for Envelope-Induced Transformation of NIH 3T3 CellsJournal of Virology, 2001
- Jaagsiekte Sheep Retrovirus Env Protein Stabilizes Retrovirus Vectors against Inactivation by Lung Surfactant, Centrifugation, and Freeze-Thaw CyclingJournal of Virology, 2001
- Sequences in the Cytoplasmic Tail of the Gibbon Ape Leukemia Virus Envelope Protein That Prevent Its Incorporation into Lentivirus VectorsJournal of Virology, 2001
- Efficient Transduction of Human Lymphocytes and CD34+ Cells via Human Immunodeficiency Virus-Based Gene Transfer VectorsHuman Gene Therapy, 1999
- Keratinocyte Growth Factor Stimulates Transduction of the Respiratory Epithelium by Retroviral VectorsHuman Gene Therapy, 1999
- In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral VectorScience, 1996
- Expanded HIV-1 Cellular Tropism by Phenotypic Mixing with Murine Endogenous RetrovirusesScience, 1990