pH standardization for phosphorus-31 magnetic resonance heart spectroscopy at different temperatures

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Abstract
Intracellular pH typically is measured by NMR using the calibrated chemical shift of the inorganic phosphate peak in phosphorus-31 spectra. Heart Spectroscopy experiments often require measurements of intracellular pH at temperatures from 5 to 37°C. This paper provides NMR pH calibrations for this range of temperatures, a summary of calibration data reported to date, and a discussion of the factors influencing pH Standardization. © 1990 Academic Press, Inc.