Allergenicity suppression in natural latex surgical gloves

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Abstract
By a revised manufacturing process we aimed to obtain latex surgical gloves without immediate allergenicity, and also to determine a simple physical technique by which to estimate this allergenicity. Five glove samples were evaluated for allergenicity by physical and biochemical methods and by skin texts at the University Allergy Centre, Three groups of allergic patients with documented anaphylaxis to surgical gloves, positive skin test to latex extract and specific IgE to latex (RAST Pharmacia & class II), volunteered for the study. The protein content, the in vitro allergenic potency of glove supernatants (RAST inhibition) and the skin test results with glove supernatants were lower in washed gloves than in non‐washed samples (P < 0.02 to P < 0.009). The supplementary effect of glove sterilization at 120°C (for 1 h in saturated steam) was obvious. The protein content became undetectable in four of the five glove supernatants, and skin tests results with sample supernatants were decreased (M±SEM = 0.68±0.29 mm vs 3.06±0.61 mm with non‐sterilized gloves), P < 0.009 by Wilcoxon test. With the Spearman test there was a significant correlation (P= 0.029) between the mean wheal size, obtained with supernatants (Groups 1 and 2) and electrical conductivity. Skin tests through pieces of gloves randomly distributed and coded were decreased by sterilization: 0.66±0.25 mm vs 2.86±00,45 mm, p 0.0001. Thus, it is possible to decrease glove allergenicity by washing them after mould forming and then sterilizing with steam. Electrical conductivity may be a simple parameter for revealing allergenicity.