HIV Type 1 Drug Resistance among Naive Patients from Venezuela
- 1 March 2007
- journal article
- research article
- Published by Mary Ann Liebert Inc in AIDS Research and Human Retroviruses
- Vol. 23 (3) , 482-485
- https://doi.org/10.1089/aid.2006.0219
Abstract
In this study, we characterize proviral DNA of 20 HIV-1 asymptomatic antiretroviral-naive patients from Venezuela in env, gag, and pol genes regions. Results from both env/gag HMA subtyping and phylogenetic analysis of pol partial sequences led to the description of clade B in all cases. Nevertheless, the high prevalence of polymorphisms was particularly evident among the protease sequences. A 10% prevalence of major resistance mutations to RTIs was found. Our data also suggested that the protease polymorphisms I62T and V77T could be considered as molecular markers of the subtype B local epidemic. In addition, we show how proviral DNA can be used as a reliable tool to follow trends of resistance mutation transmission.Keywords
This publication has 15 references indexed in Scilit:
- Trends of HIV-1 molecular epidemiology in Venezuela: introduction of subtype C and identification of a novel B/C mosaic genomeJournal of Clinical Virology, 2005
- Assessment of drug resistance mutations in plasma and peripheral blood mononuclear cells at different plasma viral loads in patients receiving HAARTJournal of Clinical Virology, 2004
- A web-based genotyping resource for viral sequencesNucleic Acids Research, 2004
- Antiretroviral Drug Resistance Testing in Adults Infected with Human Immunodeficiency Virus Type 1: 2003 Recommendations of an International AIDS Society–USA PanelClinical Infectious Diseases, 2003
- Molecular Epidemiology of HIV-1 in Venezuela: High Prevalence of HIV-1 Subtype B and Identification of a B/F Recombinant InfectionJAIDS Journal of Acquired Immune Deficiency Syndromes, 2003
- HIV Type 1 Diversity in France, 1999–2001: Molecular Characterization of non-B HIV Type 1 Subtypes and Potential Impact on Susceptibility to Antiretroviral DrugsAIDS Research and Human Retroviruses, 2003
- Analysis of HIV Type 1 Protease and Reverse Transcriptase Sequences from Venezuela for Drug Resistance-Associated Mutations and Subtype Classification: A UNAIDS StudyAIDS Research and Human Retroviruses, 2001
- Substantial Correlation between HIV Type 1 Drug-Associated Resistance Mutations in Plasma and Peripheral Blood Mononuclear Cells in Treatment-Experienced PatientsAIDS Research and Human Retroviruses, 2000
- Simplified Strategy for Detection of Recombinant Human Immunodeficiency Virus Type 1 Group M Isolates by gag/env Heteroduplex Mobility AssayJournal of Virology, 2000
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994