Genetic Characterization of the CarotenoidBiosynthetic Pathway in Methylobacterium extorquens AM1 andIsolation of a ColorlessMutant
Open Access
- 1 December 2003
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 69 (12) , 7563-7566
- https://doi.org/10.1128/aem.69.12.7563-7566.2003
Abstract
Genomic searches were used to reconstruct the putative carotenoid biosynthesis pathway in the pink-pigmented facultative methylotroph Methylobacterium extorquens AM1. Four genes for putative phytoene desaturases were identified. A colorless mutant was obtained by transposon mutagenesis, and the insertion was shown to be in one of the putative phytoene desaturase genes. Mutations in the other three did not affect color. The tetracycline marker was removed from the original transposon mutant, resulting in a pigment-free strain with wild-type growth properties useful as a tool for future experiments.Keywords
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