Binding of integration host factor (IHF) to the ilvGp1 promoter of the ilvGMEDA operon of Escherichia coli K12
- 10 July 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 16 (13) , 5973-5989
- https://doi.org/10.1093/nar/16.13.5973
Abstract
Crude extracts of Escherichia coli K-12 were found to bind DNA restriction fragments containing ilvGp1. Our analysis using a series of restriction fragments and a BamHI linker mutation indicate that a factor binds to ilvGp1 or adjacent to it. Analysis with mutant strains of E. coli K-12 and purified IHF indicate that IHF binds to ilvGp1. Furthermore, both analysis in vivo and in vitro indicate that IHF precludes transcription from ilvGp1.This publication has 25 references indexed in Scilit:
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