Partial P1 Nuclease Digestion as a Probe of tRNA Structure
- 1 June 1982
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 124 (3) , 471-476
- https://doi.org/10.1111/j.1432-1033.1982.tb06617.x
Abstract
Partial [yeast] P1 nuclease digestion of 5''-32P-labeled tRNA, followed by polyacrylamide gel electrophoresis and autoradiography, results in a series of bands of unequal intensities. Comparison of digestion profiles of 2 conformers of yeast .**GRAPHIC**. shows that P1 nuclease is sensitive to structural features of its substrate. Examination of the digestion pattern of yeast tRNAPhe in light of its known 3-dimensional conformation shows that the enzyme''s activity reflects the primary, secondary and tertiary levels of tRNA structure.This publication has 23 references indexed in Scilit:
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