Evaluation of commercially available ELISA test kits for the detection of clenbuterol and otherβ2‐agonists

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Abstract
This article presents the results of a comparative study of nine commercially available ELISA test kits for clenbuterol and other β2‐agonists currently being used in veterinary drug residue control of live and slaughtered animals in the European Union (EU). By determining measuring ranges, B/B o ‐50% values, inter‐ and intra‐assay variations and cross‐reactions as well as limits of detection (LOD) and recoveries for clenbuterol in bovine urine it could be demonstrated that a number of test kits showed considerable quality defects, reducing their applicability to residue control. LODs for urine assayed without a previous clean‐up (as recommended by all kit manufacturers) varied between 1.2 and 11.1 ng clenbuterol per ml urine and did thus not meet the requirement of 1 ng/ml of the official residue control plans of the EU Member States.