Sarcolemmal phospholamban is phosphorylated in isolated rat hearts perfused with isoprenaline
- 14 November 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 163 (2) , 297-302
- https://doi.org/10.1016/0014-5793(83)80839-4
Abstract
Phosphorylation of phospholamban in cardiac sarcolemma is implicated in the increased influx of Ca2+ through the slow calcium channel induced by catecholamines. A method is described for the preparation of highly purified sarcolemmal vesicles from rat heart, and this has been used to examine the phosphorylation of phospholamban in 32Pi-perfused rat hearts. Phospholamban phosphorylation is increased 3-fold after 30 s of perfusion with 0.1 μM isoprenaline. The time course of this increase precedes the inotropic response by 5–10 sKeywords
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