Detection of transferable R plasmids in strains of the fish–pathogenic bacterium, Pasteurella piscicida

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Abstract
Sixty strains of Pasteurella piscicida were collected from cultured yellow–tail, Seriola quinqueradiata Temminck & Schlegel, in various districts of Japan. These strains were tested for their sensitivity to 14 different chemotherapeutic agents and the detected drug resistant strains were investigated to determine whether or not they possessed R plasmids. All strains were most susceptible to ampicillin of the tested drugs. All except five strains were found to be susceptible to chloramphenicol (CM) and doxycycline (DOTC). However, the strains were only moderately susceptible to cephazolin, cephalexin, nalidixic acid, trimethoprim and ormethoprim, and their MIC values were under 3–lμg/ml. Twenty–one strains showed resistance to furazolidone (NF). Five out of the 60 strains were resistant to CM, tetracycline (TC), kanamycin (KM), NF and sulphamonomethoxine (SA). Transferable R plasmids were detected in these drug–resistant strains. These R plasmids had markers for resistance to CM, TC, KM and SA.