Fibroblast products adsorb to the culture substrate and stimulate growth of human umbilical vein endothelial cells

Publisher Website
Google Scholar
Abstract
Human umbilical vein endothelial cells grew with a doubling time of approximately 20 hr in medium conditioned by human diploid fibroblasts and supplemented with 10% fetal bovine serum, whereas the cells did not grow substantially in the non‐conditioned serum supplemented medium. Production of the fibroblast‐derived activity required the presence of insulin, EGF, or PDGF. The fibroblast derived‐factor adsorbed to native culture dishes or dishes coated with gelatin and collagen. The adsorbed activity was resistant to treatment with 1% Triton X‐100, and was abolished by treatment with serine proteases. Further, the extracellular matrix produced by the fibroblasts also showed growth‐stimulating activity. Fibroblast‐derived factors may play a role in vascularization processes during wound healing, inflammation and normal development.