Human Factor VIII: Morphometric Analysis of Purified Material in Solution

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Abstract
Study of purified human factor VIII in buffer by freeze-etch electron microscopy reveals rounded, rod-shaped particles measuring 22 by 42 nanometers. When thrombin was added to purified normal factor VIII, there was a rapid loss of rod-shaped particles during the first 15 minutes of incubation at 37°C. Purified plasma from two patients with severe hemophilia contained spherical particles measuring 10 to 50 nanometers in diameter, with no evidence of significant numbers of rod-shaped forms. Negatively stained and unstained air-dried samples of factor VIII corroborate the relative shape and size differences between normal and hemophiliac material.