Programmed Adsorption and Release of Proteins in a Microfluidic Device
Top Cited Papers
- 18 July 2003
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 301 (5631) , 352-354
- https://doi.org/10.1126/science.1080759
Abstract
A microfluidic device has been developed that can adsorb proteins from solution, hold them with negligible denaturation, and release them on command. The active element in the device is a 4-nanometer-thick polymer film that can be thermally switched between an antifouling hydrophilic state and a protein-adsorbing state that is more hydrophobic. This active polymer has been integrated into a microfluidic hot plate that can be programmed to adsorb and desorb protein monolayers in less than 1 second. The rapid response characteristics of the device can be manipulated for proteomic functions, including preconcentration and separation of soluble proteins on an integrated fluidics chip.Keywords
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