Responses of GPCR135 to Human Gene 3 (H3) Relaxin in CHO‐K1 Cells Determined by Microphysiometry
- 1 May 2005
- journal article
- Published by Wiley in Annals of the New York Academy of Sciences
- Vol. 1041 (1) , 332-337
- https://doi.org/10.1196/annals.1282.053
Abstract
This study examined the functional response to human relaxin 2 (H2 relaxin), human relaxin 3 (H3 relaxin), porcine relaxin, and human INSL3 in the cytosensor microphysiometer, using CHO-K1 cells stably expressing human GPCR135. CHO-K1 cells stably expressing GPCR135 were generated by the serial dilution method and receptor properties were assessed. Saturation studies of [125I] H3 relaxin binding to GPCR135 in these cells gave a Bmax of 32.61 +/- 6.5 fmol/mg protein and Kd of 0.12 +/- 0.08 nM. The functional response to H3 relaxin and other relaxin/insulin peptides of GPCR135 expressed in CHO-K1 cells was measured in the cytosensor microphysiometer and analyzed using inhibitors of signal transduction proteins.Keywords
This publication has 5 references indexed in Scilit:
- Pharmacological Characterization of Relaxin-3/INSL7 Receptors GPCR135 and GPCR142 from Different Mammalian SpeciesThe Journal of Pharmacology and Experimental Therapeutics, 2005
- Identification of Relaxin-3/INSL7 as an Endogenous Ligand for the Orphan G-protein-coupled Receptor GPCR135Journal of Biological Chemistry, 2003
- The novel G-protein coupled receptor SALPR shares sequence similarity with somatostatin and angiotensin receptorsGene, 2000
- Identification of Distinct Signalling Pathways for Somatostatin Receptors SSTR1 and SSTR2 as Revealed by MicrophysiometryCellular Signalling, 1999
- Purification and characterization of porcine prorelaxinJournal of Biochemical and Biophysical Methods, 1996