A Quantitative Study of Passive Anaphylaxis in the Guinea-Pig II
Open Access
- 1 March 1944
- journal article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 48 (3) , 181-183
- https://doi.org/10.4049/jimmunol.48.3.181
Abstract
In a previous communication (1) it was shown that intravenous injection of 0.03 milligram of rabbit anti-ovalbumin nitrogen or Type III antipneumococcal antibody-nitrogen would passively sensitize a 250 gram guinea-pig so that fatal anaphylaxis resulted on subsequent intravenous injection of 1 milligram of ovalbumin or 0.10 milligram of Type III specific polysaccharide respectively. No studies were carried out with guinea-pig antibody in passive anaphylaxis since precipitating guinea-pig sera were not available. However, the method developed by Freund and McDermott (2), employing adjuvants in immunization, results in guinea-pig antisera with amounts of antibody adequate for analysis by the quantitative precipitin-method (3) and for anaphylactic studies. In general, the same quantitative relations were found to obtain for guinea-pig antibody as had been established for rabbit antibody (1). Experimental. Guinea-pig antibodies to ovalbumin were used to produce passive anaphylaxis.Keywords
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