Expression of the Drosophila 70 000 Dalton heat shock protein is translationally controlled in yeast

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Abstract
Plasmid pPW229, containing the 2·25 kilobase transcribed sequence for the 70 000 Dalton heat shock protein of Drosophila,1 was integrated into plasmid CV13 and used to transform Saccharomyces cerevisiae. Upon a heat shock, at 41°C for 20 min, a new 70 000 Dalton protein appeared in the transformants. This protein was not detected in transformants grown at 23°C, nor in transfromants carrying the hybrid plasmid from which the structural gene for the 70 000 Dalton protein had been deleted. RNA was isolated from transromants grown at 23°C and from transformants heat shocked at 41°C. RNA complementary to the Drosophila heat shock gene was present in the transformants, grown either at 23°C or heat shocked. No complementary RNA was detected in yeast cells transformed with the hybrid plasmid from which the structural gene had been deleted. The Drosophila heat shock gene in yeast appears to be transcribed constitutively but translated only under heat shock conditions.