Molecular cloning of rat Krev-1 cDNA and analysis of the mRNA levels in normal and NMU-induced mammary carcinomas

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Abstract
Human Krev-1 has been reported to reverse the transformed phenotype in a variety of cell types containing activated ras. Most mammary carcinomas induced by N-nitrosomethylurea in the rat have been reported to exhibit activated ras. Here we report that rat Krev-1 has a very high degree of homology to human Krev-1. Furthermore, Krev-1 expression is high in both normal and neoplastic mammary glands. No difference was found in expression levels in tumors with and without ras activation. These data argue against a tumor suppressor function for Krev-1 in the in vivo rat mammary gland.