A Method for in vitro Studies on Fluoride Uptake in Enamel Using Single Tooth Surfaces

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Abstract
A method for in vitro studies of fluoride uptake in single tooth surfaces has been developed. Two symmetrically situated areas of identical size are isolated on buccal surfaces of premolars by means of a special wax casting method. Analysis of 23 teeth demonstrated that the average fluoride concentration in the two areas at the same distance from the enamel surface was almost identical. The correlation between the fluoride concentration of the compared two areas of the same tooth, located at the same distance from the enamel surface, proved to be least in the outermost layer (r = 0.7). In more deeply situated layers the correlation coefficient was approximately 0.9. Calculation of the difference in the fluoride concentration at predetermined distances from the enamel surface by means of a computer program makes possible an evaluation of fluoride uptake under various experimental conditions.

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