Production and Characterization of a Monoclonal Antibody Against Mannose-Sensitive Hemagglutinin ofVibrio cholerae

1 February 1998

journal article
research article
Published by Mary Ann Liebert Inc in Hybridoma

Vol. 17 (1) , 63-67
https://doi.org/10.1089/hyb.1998.17.63

Abstract

We have generated murine monoclonal antibodies (MAb) against Vibrio cholerae mannose-sensitive hemagglutinin (MSHA) using conventional hybridoma procedures. Seven hybridomas were obtained and one characterized. Hybridoma 2F12/F1 secreted an antibody of the IgG₃ type that reacted with a 17-kDa antigen corresponding to the product of the mshA gene. This MAb inhibited mannose-sensitive agglutination of chicken erythrocytes by EL tor and O139 vibrios. Vibrios expressing MSHA activity inhibited binding of the antibody secreted by 2F12/F1 to MSHA-coated microtiter plates.

Keywords

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