Transplantation to the rat brain of human neural progenitors that were genetically modified using adenoviruses
- 1 March 1995
- journal article
- Published by Springer Nature in Nature Genetics
- Vol. 9 (3) , 256-260
- https://doi.org/10.1038/ng0395-256
Abstract
Transplantations for neurological disorders are limited by the supply of human fetal tissue. To generate larger numbers of cells of appropriate phenotype, we investigated whether human neural progenitors expanded in vitro could be modified with recombinant adenoviruses. Strong expression of beta-galactosidase was obtained in vitro. Two or three weeks after transplantation of engineered cells to the rat brain, we observed a small percentage of surviving neuroblasts strongly expressing beta-galactosidase in four out of 13 rats. Thus human precursor cells that have been genetically modified using adenoviruses are a promising tool for ex vivo gene therapy of neurodegenerative diseases.Keywords
This publication has 35 references indexed in Scilit:
- Development of endogenous beta-galactosidase and autofluorescence in rat brain microvessels: implications for cell tracking and gene transfer studies.Journal of Histochemistry & Cytochemistry, 1994
- Inactivation of E2a in recombinant adenoviruses improves the prospect for gene therapy in cystic fibrosisNature Genetics, 1994
- Better cells for brain repairNature, 1993
- Long-term correction of rat model of Parkinson's disease by gene therapyNature, 1993
- Generation of Neurons and Astrocytes from Isolated Cells of the Adult Mammalian Central Nervous SystemScience, 1992
- Survival and function of intrastriatally grafted primary fibroblasts genetically modified to produce l-dopaNeuron, 1991
- Cell lineage in the cerebral cortex of the mouse studied in vivo and in vitro with a Recombinant RetrovirusNeuron, 1988
- Identical reactivity of monoclonal antibodies HNK-1 and NC-1: conservation in vertebrates on cells derived from the neural primordium and on some leukocytesCell Differentiation, 1984
- Developmental profile of three enolase isozymes in rat brain determination from one-cell embryo to adult brainNeurochemistry International, 1984
- Estimation of nuclear population from microtome sectionsThe Anatomical Record, 1946