The plasma membrane Ca2+ pump from proximal kidney tubules is exclusively localized and active in caveolae
- 11 September 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 576 (1-2) , 31-35
- https://doi.org/10.1016/j.febslet.2004.08.055
Abstract
Plasma membrane Ca2+-ATPase is involved in the fine-tuned regulation of intracellular Ca2+. In this study, the presence of Ca2+-ATPase in caveolae from kidney basolateral membranes was investigated. With the use of a discontinuous sucrose gradient, we show that Ca2+-ATPase is exclusively located and fully active in caveolin-containing microdomains. Treatment with methyl-β-cyclodextrin – a cholesterol chelator – leads to a spreading of both caveolin and completely inactive Ca2+-ATPase toward high-density fractions. These data support the view that Ca2+ fluxes mediated by Ca2+-ATPase in kidney epithelial cells occur only in caveolae, being strictly dependent on the integrity of these microdomainsKeywords
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