Reproducibility of phase‐contrast microscope measurements of percentage motile microorganisms in samples removed from the dorsum of the tongue

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Abstract
The purpose of this investigation was to determine the accuracy of measurements of percentage motile microorganisms in samples removed from the dorsum of the tongue, using a phase‐contrast microscope. The results indicated that the percentage of motile microorganisms in a bacterial sample can be accurately assessed provided that a short interval of time elapses between sampling and measuring. Furthermore, it appeared that the percentage of motile microorganisms varied during the course of the day.