Abstract
In many plant species, particularly those of agricultural importance, there is now much effort being devoted to developing comprehensive genetic maps using biochemical and molecular markers. Because these techniques often involve destructive sampling of individual plants the use is increasingly made of homozygous or near-homozygous recombinant lines for linkage studies in preference to F2 or backcross generations. The present paper describes methods for the detection and estimation of linkage using such generations for commonly encountered genetic situations.