The Isolation and Pathogenicity of Pityrosporum ovale

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Abstract
P. ovale was repeatedly and easily isolated by planting scales from seborrhea oleosa in acid dextrose broth containing 23-44% glycerin and incubating at 30-37[degree]C. Subcultures were successfully carried on media prepared by pipetting ether extract of lanolin, oleic acid, or seborrheic scales over agar slants, as descr. by Benham. Evidence that the organism was actually P. ovale was furnished by the necessity for using special media, the resemblance of the fungus in culture and in the skin, and a series of observations of the inoculum which showed a continuity of development of the cells of P. ovale in the scales.

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