Abstract
Various enzymes in the semen of men were examined to see if any could be related to measures of fertility. Fumarase [EC 4.2.1.2] activity was highly correlated with sperm number and percentage motility. Diamine oxidase [EC 1.4.3.6] activity was higher in samples with sperm counts of < 20 .times. 106/ml and sperm motility of < 20%. Monoamine oxidase, adenine deaminase and prostaglandin dehydrogenase were undetectable in significant amounts in all samples, while peroxidase and adeonosine deaminase were not correlated with sperm count and motility. The simple spectrophotometric assays for fumarase and diamine oxidase could form the basis of a routine assessment of human semen samples for estimation of male infertility.

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