Studies on the Isolation and Chemical-Physical Properties of Porcine Follicle-Stimulating Hormone*

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Abstract
Methods are described for isolating highly purified FSH [follicle stimulating hormone] from porcine pituitary glands. The biological activity of pure material was 22 NIH[National Institute of Health]-FSH-P2 U[units]/mg, as judged by the ovarian weight augmentation bioassay. Virtually no immunoreactive LH [luteinizing hormone] was indicated by RIA [radioimmunoassay], and only 1 component was detected by both gel electrophoresis and immunoprecipitation. Amino acid and carbohydrate analyses of the highly purified FSH indicated the presence of 1-tryptophan, a high content of aspartic and glutamic acids, and 6% sialic acid. MW of untreated and guanidine-treated porcine FSH were estimated from hydrodynamic measurements of Stokes radii and sedimentation coefficients. A highly specific and sensitive RIA was also developed for this hormone.

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