Hygromycin Resistance Gene Cassettes for Vector Construction and Selection of Transformed Rice Protoplasts
- 1 October 1991
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 97 (2) , 832-835
- https://doi.org/10.1104/pp.97.2.832
Abstract
Hygromycin resistance gene cassettes were designed to facilitate vector construction for plant transformation. Unique EcoRI, Pstl, and Sacll sites in the coding sequence of a hygromycin B phosphotransferase gene (hph) from Escherichia coli were eliminated. The mutated hph genes were used to form gene cassettes flanked by EcoRI-Sacll-Kpnl-Hindlll sites. Hygromycin resistance of wild-type and mutated hph genes was indistinguishable in E. coli and rice protoplast growth assay.Keywords
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