Functional Expression of GABAA Chloride Channels and Benzodiazepine Binding Sites in Baculovirus Infected Insect Cells
- 1 June 1992
- journal article
- Published by Springer Nature in Nature Biotechnology
- Vol. 10 (6) , 679-681
- https://doi.org/10.1038/nbt0692-679
Abstract
We have employed the baculovirus expression system for the production of insect cell membranes having GABA/benzo–diazepine binding sites. Three recombinant baculoviruses each harboring a different GABAA receptor cDNA were introduced into insect cells by simultaneous infection. Infected cells expressed GABA responsive Cl− channels and benzodiazepine binding sites with the same pharmacological specificity as animal cells expressing these receptor subunits.Keywords
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